Yoshua Donny Rudinatha, S.Si., M.Si., Ph.D.

Research Interest

Metabolic Engineering
Yeast Engineering
Metabolic/Kinetic Modelling
CRISPR-Cas9
Synthetic Biology

Biography

Yoshua Donny Rudinatha, S.Si., M.Si., Ph.D. completed his bachelor’s degree in Chemistry at the Bandung Institute of Technology (ITB), where he continued in an honors and fast-track program to obtain his master’s degree in Chemistry (Biochemistry) the following year. As part of the Sandwich program, he conducted his master’s thesis research at the University of Groningen, the Netherlands.

He pursued his Ph.D. at the University of Natural Resources and Life Sciences (BOKU), Vienna, Austria, in the BioToP (Biomolecular Technology of Proteins) program with funding from the Austrian Science Fund (FWF). His research has consistently focused on yeast, particularly in the areas of yeast engineering and metabolic engineering using the CRISPR system.

During his Ph.D., he studied glycolysis in Komagataella phaffii (formerly Pichia pastoris), characterizing the kinetic parameters of glycolysis and fermentation enzymes. He developed kinetic models for each enzyme and subsequently conducted global metabolic engineering of the glycolysis pathway using CRISPR/Cas9 to investigate the Crabtree phenotype. Utilizing next-generation sequencing techniques such as whole genome sequencing (WGS) and RNA-Seq, he and his team successfully characterized the newly engineered strain.

Award

Year	Award/Grant Name	Source of Award
2019	BioToP Ph.D Program	FWF (Austrian Science Fund)
2018	Sandwich Program ITB	ITB

Selected Publication

Nijland, J. G., Shin, H. Y., Dore, E., Rudinatha, D., de Waal, P. P., & Driessen, A. J. M. (2021). D-glucose overflow metabolism in an evolutionary engineered high-performance D-xylose consuming Saccharomyces cerevisiae strain. Fems Yeast Research, 21(1), [foaa062].