IVANNA, M.Sc.
FACULTY OF BIOMEDICINE

In 2012, she was awarded the highly competitive Erasmus Mundus Scholarship and joined the Interuniversity Program of Molecular Biology, of the Katholieke Universiteit Leuven, Antwerpen Universiteit and Vrije Universiteit Brussel (VUB), Belgium. She worked with Reproduction and Genetic research group of the VUB during the academic year of 2013-2014 on her master thesis ‘DNA Damage in hESC Cultured on Feeder-free Conditions: The Impact of Cell Density’. After completing her Master study, she became a Research Assistant at the Institute of Atomic and Molecular Science, National Taiwan University, and worked on understanding structures and functions of primary cilia, an important organelle essential for key cell signaling pathways, including sonic hedgehog signaling, non-canonical Wnt signaling, and PDGFα signaling, in mammalian and stem cells.

 

RESEARCHES AND PROJECTS

Tracing Genetic Ancestry of Peranakan in Singapore, 2017–present 

  • The project is a collaborative effort between i3L and Genome Institute Singapore (GIS).
  • The project aims to perform an admixture analysis of the Peranakan genomes that will enable us to depict the history of this special community from a unique angle solely based on genetic data. Peranakan Chinese are the descendants of Chinese who intermarried with the Malays when they were immigrants who came to the Malay Archipelago between the 15th and 17th centuries.

The effect of RPGRIP1L knockdown to the development of primary cilia in retinal pigment epithelial (RPE) cells, 2015

  • The project was carried out while working as a Research Assistant in Institute of Atomic and Molecular Science in Taiwan.
  • RPGRIP1L is a ciliary transition zone protein and we hypothesized that it is one of the core structural components of primary cilia’s transition zone. If there is no RPGRIP1L, other ciliary protein will not be recruited and thus primary cilia will be absent from the cell.
  • Skills learned: immunostaining, fluorescence microscopy, molecular cloning, qRT-PCR, RT-PCR, cell culture, siRNA transfection, protein & RNA extraction.

The effect of hypoxic condition to the ciliation frequency of RPE cells, 2015

  • The project was carried out while working as a Research Assistant in Institute of Atomic and Molecular Science in Taiwan.
  • It was hypothesized that hypoxic condition will inhibit ciliogenesis. However, no data supported this hypothesis.
  • Skills learned: immunostaining, fluorescence microscopy, molecular cloning, qRT-PCR, RT-PCR, cell culture, protein & RNA extraction.

DNA damage in human embryonic stem cell (hESC) cultured on feeder-free conditions: the impact of cell density, 2013–2014

  • The project was carried out in partial fulfillment of the requirements for the Degree of Master of Science in Molecular Biology.
  • The correlation between cell density and DNA breaks in hESC kept in a feeder-free culturing system was investigated.
  • Skills learned: immunostaining, fluorescence microscopy, comet assay, FACS, stem cell culturing system.

Identification and Optimization of Indigenous Probiotic Bacteria Against Vibrio Harveyi Isolated from Hatchery ofLitopenaeus vannameiCulture in Situbondo, East Java 2010–2011

  • The project was carried out in partial fulfillment of the requirements for the Degree of Bachelor of Science in Microbiology.
  • Inhibitory activity of marine bacterium – Halomonas aquamarina and Shewanella algae – isolated from hatchery water in Situbondo against pathogenic effects of Vibrio Harvey were studied. Research was done to find out the optimum condition for inhibition of V.harveyi and the determination of antimicrobial’s spectrum.
  • Skills learned: microbiology, Kirby-Bauer method, Minimum Inhibitory Concentration determination.

RESEARCH PUBLICATIONS

  • Williantarra I. (2018). Pengaruh Protein RPGRIP1L pada Pembentukan Silia Primer sebagai Kandidat Target Terapi Gen Penyakit Siliopati.Cermin Dunia Kedokteran. Jakarta: Kalbemed.
  • Williantarra I. (2017). Source of Genetic Aberrations in Human Embryonic Stem Cell: Common Fragile Sites and Replication Stress.Cermin Dunia Kedokteran. Jakarta: Kalbemed.
  • Ivanna. (2014). DNA Damage in hESC Cultured on Feeder-free Condition: The Impact of Cell Density. Dissertation for Master of Science. Research Group Reproduction and Genetic, Vrije Universiteit Brussel. Promoted by Prof. Dr. Claudia Spits.
  • Ivanna. (2011). Identification and Optimization ofHalomonas aquamarinaAgainstVibrio HarveyiIsolated from Hatchery of Litopenaeus vannamei Culture in Situbondo, East Java. Thesis for Bachelor of Science. Microbiology, Genetic and Molecular Biology Research Group, School of Life Sciences and Technology, Institut Teknologi Bandung. Promoted by Dr. Pingkan Aditiawat